李惠春老師實驗室介紹

專長：蛋白質化學、蛋白質結構與功能

研究主題: 探討C型肝炎病毒套膜蛋白(E1、E2)結構與功能之關係

說明:
許多動物病毒外都包覆了一層由宿主細胞膜所衍生的膜，膜上分佈有病毒的醣蛋白，有些外套膜上的醣蛋白協助辨識宿主細胞。當病毒被宿主細胞吞噬初期會形成vesicle，病毒的外套膜便會與vesicle的膜融合，而將病毒的遺傳物質釋放至宿主細胞的細胞質中。此膜融合的過程便是由病毒套膜上的醣蛋白所負責的，C肝病毒的E1及E2蛋白疑似具有這樣的功能。目前，C肝病毒的E1及E2蛋白被歸類為第二型膜融合蛋白，即先以dimmers型式存在，受酸鹼值改變而形成trimer，再藉由構型改變使兩層膜融合為一。只是在C肝病毒中，此過程的分子機制及蛋白質構形變化尚不明瞭。

我們利用E. coli 大量表達並純化C肝病毒的E1及E2蛋白，配合生化及生物物理的方法來探討它們在膜融合過程中的角色，尤其是參與膜融合的序列、在膜融合前與融合後的蛋白構形、及因酸鹼值改變所引起的構形改變。

最近發表之論文:
1. Bertrand Vileno, Jean Chamouna, Hua Liang, Paul Brewerc, Brian D. Haldemanc, Kevin C. Facemyer, Bridget Salzameda, Likai Song, Hui-Chun Li, Christine R. Cremo, and Piotr G. Fajer. 2011. Broad disorder and the allosteric mechanism of myosin II regulation by phosphorylation. Proc Natl Acad Sci USA. 108:8218-23.

2. Shih-Ching Chan, Shih-Yen Lo, Je-Wen Liou, Min-Ching Lin, Ciao-Ling Syu, Meng-Jiun Lai , Yi- Cheng Chen, Hui-chun Li. 2011. Visualization of the structures of the hepatitis C virus replication complex. Biochem. Bioph. Res. Co. 404:574-578.

3. Chee-Hing Yang, Hui-Chun Li, Jeng-Geng Jiang, Che-Fang Hsu, Yi-Jen Wang, Meng-Jiun Lai, Yue-Li Juang and Shih-Yen Lo. 2010. Enterovirus type 71 2A protease functions as a transcriptional activator in yeast. J. Biomed Sci. 17:65.

4. Chia-Wei Chang, Hui-Chun Li, Che-Fang Hsu, Chiao-Yen Chang and Shih-Yen Lo. 2009. Increased ATP generation in the host cell is required for efficient vaccinia virus production. J. Biomed Sci. 16:80-89.

5. Shih-Chi Chen, Shih-Yen, Lo, Hsin-Chieh, Ma, Hui-Chun Li. 2009. Expression and membrane integration of SARS-CoV E protein and its interaction with M protein. Virus Genes 38:365-371.

6. Yi-Ching Hsieh, Hui-Chun Li, Shih-Chi Chen and Shih-Yen Lo. 2008. Interactions between M protein and other structural proteins of severe, acute respiratory syndrome-associated coronavirus. J. Biomed Sci. 15:707-717.

7. Hsin-Chieh Ma, Ta-Wei Lin, Hui-Chun Li, Sanae M.M. Iguchi-Ariga, Hiroyoshi Ariga, Yu-Li Chuang, Jing-Hsiung Ou, Shih-Yen. 2008. Hepatitis C virus ARFP/F protein interacts with cellular MM-1 protein and enhances the gene trans-activation activity of c-Myc. J. Biomed Sci. 15:417-425.

8. Hsin-Chieh Ma, Chiu-Ping Fang, Yi-Ching Hsieh, Shih-Chi Chen, Hui-Chun Li, Shih-Yen Lo. 2008. Expression and membrane integration of SARS-CoV M protein. J. Biomed Sci. 15:301-310.

9. Li, H.-C., Song, L., Salzameda, B., Cremo, C. R. Fajer, P. G. 2006. Rugulatory and Catalytic Domain Dynamics of Smooth Muscle Myosin Filaments. Biochemistry 45;6212-6221.