大家都忙碌了一學期， 7/15中午我們去好好吃一餐！
2011年7月14日 星期四
2011年7月10日 星期日
2011年7月6日 星期三
2011年6月15日 星期三
李展平老師實驗室介紹
專長：生物化學、分子生物學、微生物分子診斷學、奈米生物醫學
研究主題: 微生物分子診斷、奈米生物技術
說明:
1. Structure-Function of Nucleic Acids and Interactions between Nucleic Acid and Protein
One of our interests is to explore a very promising field of analyzing structural features of nucleic acids and nucleic acids-proteins interactions by molecular/biochemical analyses and direct image analyses using Scanning Probe Microscopy (SPM). We are in collaboration with Dr CC Wang’s group; they are specialized in the SPM technologies, at NCKU. Our molecular/ biochemical efforts are to clone the protein genes including RecA, SSB, T7 endonuclease I, pyrophosphatase, lambda integrase, P1 cre recombinase, Q Beta replicase, and many others, to express them in E. coli cells, to purify them with high purity and activity, and to analyze their functions in vitro and in vivo. DNA or RNA with specialized structural elements such as cruciform, H-DNA, attB/P site, pseudoknot will also be cloned or constructed. We expect these results (molecular/chemical and SPM) will provide us deeper insights into structural features of nucleic acids and even the dynamically structural changes under different conditions and also after interactions with proteins.
2. Molecular diagnosis of diseases caused by pathogens
For the past years, we have been focusing on the development of methods for the molecular diagnosis of diseases cause by pathogens, such as MTB, four blood-born viruses (HBV, HCV, HIV, and HTLV, assay performance see Figure 2) and many others. At the same period, we have also developed the method of using nano si-coated magnetic beads for the purification of viral DNA/RNA from clinical samples with low virus titer; cloned, expressed and purified PCR enzymes and related proteins to establish many PCR and RT-PCR assays. In addition, related technologies such as the use of nanogold for the detection of mutations and a new technology platform that would allow us to carry out high throughput screening of mutations and SNP, and also for genotyping analyses.
研究主題: 微生物分子診斷、奈米生物技術
說明:
1. Structure-Function of Nucleic Acids and Interactions between Nucleic Acid and Protein
One of our interests is to explore a very promising field of analyzing structural features of nucleic acids and nucleic acids-proteins interactions by molecular/biochemical analyses and direct image analyses using Scanning Probe Microscopy (SPM). We are in collaboration with Dr CC Wang’s group; they are specialized in the SPM technologies, at NCKU. Our molecular/ biochemical efforts are to clone the protein genes including RecA, SSB, T7 endonuclease I, pyrophosphatase, lambda integrase, P1 cre recombinase, Q Beta replicase, and many others, to express them in E. coli cells, to purify them with high purity and activity, and to analyze their functions in vitro and in vivo. DNA or RNA with specialized structural elements such as cruciform, H-DNA, attB/P site, pseudoknot will also be cloned or constructed. We expect these results (molecular/chemical and SPM) will provide us deeper insights into structural features of nucleic acids and even the dynamically structural changes under different conditions and also after interactions with proteins.
2. Molecular diagnosis of diseases caused by pathogens
For the past years, we have been focusing on the development of methods for the molecular diagnosis of diseases cause by pathogens, such as MTB, four blood-born viruses (HBV, HCV, HIV, and HTLV, assay performance see Figure 2) and many others. At the same period, we have also developed the method of using nano si-coated magnetic beads for the purification of viral DNA/RNA from clinical samples with low virus titer; cloned, expressed and purified PCR enzymes and related proteins to establish many PCR and RT-PCR assays. In addition, related technologies such as the use of nanogold for the detection of mutations and a new technology platform that would allow us to carry out high throughput screening of mutations and SNP, and also for genotyping analyses.
江信仲老師實驗室介紹
專長：細胞生物學、血管生物學、分子生物學
研究主題：1. CD93 對內皮細胞發炎的影響
2. 研究microRNA做為治療模式鼠視網膜新生血管病變潛在目標的
應用，以及對組織內部分子表達及新生血管形成的影響
說明:
血管內皮細胞（vascular endothelial cells）是位於血管最內層、與血液直接接觸的地方，具有調節血管緊張度、血管通透性及促進血管發生的作用，對於調控血管功能扮演重要角色，在調節血液流變性、血球細胞對血管壁粘附等方面也發揮關鍵作用。因此，內皮細胞功能障礙被認為是慢性血管併發症諸如糖尿病及心血管動脈硬化的關鍵因素。在正常情況下，白血球在血管中流動，並不會與血管內皮細胞黏著。但當體內有外來物入侵或發炎反應產生時，因組織受損，導致血管內皮細胞受到細胞刺激 (如TNF-α、LPS、IL-1、IL-6等)作用之下，影響血管內微環境的發炎反應。而發炎反應的發生乃藉由血管內皮細胞表現黏著分子（如ICAM-1及VCAM-1），促使循環白血球黏著到血管壁上，進一步移行到發炎組織部位，執行免疫反應。若血管內皮的屏障和通透性改變，使氧化低密度膽固醇增加，此變性的氧化低密度膽固醇，會刺激產生化學趨性物質，吸引血液中的單核球進入內皮層的內皮細胞下空隙，並轉變成巨噬細胞，將氧化低密度膽固醇吞噬，而反覆吞噬氧化低密度膽固醇的巨噬細胞，則終至變成泡沬細胞沈積在內皮層內。若此情形繼續進行，則一而再，再而三的巨噬細胞形成的泡沬細胞死亡而疊積，再加上結締組織增生與修補，則將造成早期的動脈硬化斑（fatty streak），若動脈硬化繼續進行，則形成動脈粥狀硬化塊（atherosclerotic plaque）。漸漸進展增大的動脈硬化塊，則會造成阻塞及狹窄之症狀，最終形成動脈硬化。CD93是一種表現在內皮細胞與單核球的C-type lectin 穿膜蛋白，之前我們的研究結果發現CD93會干擾血管內膽固醇的表現，在動物實驗中會促進血管不正常栓塞，因此研究CD93對刺激膽固醇表現的機制是本實驗室的主題之一。
早產兒視網膜病變(Retinopathy of Prematurity)是一種發生於早產兒或低體重新生兒的視網膜病變。主要是因為早產兒出生時視網膜尚未發育完全， 加上出生後在保溫箱內受到高濃度氧氣的刺激，先天不良與後天失調，造成視網膜血液供應系統失衡。輕者局部視網膜缺氧，重者大量不正常血管滋生，造成出血，甚至視網膜剝離，導至失明。血管新生(angiogenesis)是正常生理變化中(如生長、傷口癒合)所必需有的過程，近年來科學家們也發現它和癌症的發展有密切的關係。當周圍的結締組織刺激後會分泌許多促使血管新生的物質(angiogenic substances)激活血管內皮細胞，而發生下列變化：1) 腫瘤周圍結締組織的分解破壞； 2) 內皮細胞增生； 3) 內皮細胞向分泌促使血管新生物質的地方移動； 4) 內皮細胞重新組合成新生血管。本實驗室另一方向在研究高濃度氧氣環境下，內皮細胞被誘導產生血管新生，用來做為病變致病機轉的研究。
最近發表之論文:
1. Liu SL, Li YH, Shi GY, Tang SH, Jiang SJ, Huang CW, Liu PY, Hong JS, Wu HL. 2009 Apr. Dextromethorphan reduces oxidative stress and inhibits atherosclerosis and neointima formation in mice. Cardiovasc Res. 82(1):161-169. (SCI)
2. Jiang SJ, Campbell LA, Berry MW, Rosenfeld ME, Kuo CC. 2008 Jul. Retinoic acid prevents Chlamydia pneumoniae induced foam cell development in a mouse model of atherosclerosis. Microbes Infect. 10(12-13):1393-1397. (SCI)
3. Jiang SJ, Kuo CC, Berry MW, Lee AW, Campbell LA. 2008 Apr. Identification and characterization of Chlamydia pneumoniae specific antigens which activateF production in RAW 264.7 murine macrophages. Infect. Immun. 76(4): 1558-1564. (SCI)
4. Kuo CC, Lee A, Jiang SJ, Yaraei K, Campbell LA. 2007 Jul. Inoculation of Chlamydia pneumoniae or Chlamydia trachomatis with ligand that inhibit attachment to host cells reduces infectivity in the mouse model of lung infection: implication for anti-adhesive therapy. Microbes Infect. 9(9):1139-1141. (SCI)
5. Jiang SJ, Lin TM, Shi GY, Eng HL, Chen HY, Wu HL. 2004 Sep. Inhibition of bovine herpesvirus-4 replication in endothelial cells by arsenite. Antiviral Res. 63(3):167-175. (SCI)
6. Jiang SJ, Lin TM, Shi GY, Eng HL, Chen HY, Wu HL. 2004 Jul-Aug. Inhibition of bovine herpesvirus-4 replication by arsenite through downregulation of the extracellular signal-regulated kinase signaling pathway. J Biomed Sci. 11(4):500-510. (SCI)
研究主題：1. CD93 對內皮細胞發炎的影響
2. 研究microRNA做為治療模式鼠視網膜新生血管病變潛在目標的
應用，以及對組織內部分子表達及新生血管形成的影響
說明:
血管內皮細胞（vascular endothelial cells）是位於血管最內層、與血液直接接觸的地方，具有調節血管緊張度、血管通透性及促進血管發生的作用，對於調控血管功能扮演重要角色，在調節血液流變性、血球細胞對血管壁粘附等方面也發揮關鍵作用。因此，內皮細胞功能障礙被認為是慢性血管併發症諸如糖尿病及心血管動脈硬化的關鍵因素。在正常情況下，白血球在血管中流動，並不會與血管內皮細胞黏著。但當體內有外來物入侵或發炎反應產生時，因組織受損，導致血管內皮細胞受到細胞刺激 (如TNF-α、LPS、IL-1、IL-6等)作用之下，影響血管內微環境的發炎反應。而發炎反應的發生乃藉由血管內皮細胞表現黏著分子（如ICAM-1及VCAM-1），促使循環白血球黏著到血管壁上，進一步移行到發炎組織部位，執行免疫反應。若血管內皮的屏障和通透性改變，使氧化低密度膽固醇增加，此變性的氧化低密度膽固醇，會刺激產生化學趨性物質，吸引血液中的單核球進入內皮層的內皮細胞下空隙，並轉變成巨噬細胞，將氧化低密度膽固醇吞噬，而反覆吞噬氧化低密度膽固醇的巨噬細胞，則終至變成泡沬細胞沈積在內皮層內。若此情形繼續進行，則一而再，再而三的巨噬細胞形成的泡沬細胞死亡而疊積，再加上結締組織增生與修補，則將造成早期的動脈硬化斑（fatty streak），若動脈硬化繼續進行，則形成動脈粥狀硬化塊（atherosclerotic plaque）。漸漸進展增大的動脈硬化塊，則會造成阻塞及狹窄之症狀，最終形成動脈硬化。CD93是一種表現在內皮細胞與單核球的C-type lectin 穿膜蛋白，之前我們的研究結果發現CD93會干擾血管內膽固醇的表現，在動物實驗中會促進血管不正常栓塞，因此研究CD93對刺激膽固醇表現的機制是本實驗室的主題之一。
早產兒視網膜病變(Retinopathy of Prematurity)是一種發生於早產兒或低體重新生兒的視網膜病變。主要是因為早產兒出生時視網膜尚未發育完全， 加上出生後在保溫箱內受到高濃度氧氣的刺激，先天不良與後天失調，造成視網膜血液供應系統失衡。輕者局部視網膜缺氧，重者大量不正常血管滋生，造成出血，甚至視網膜剝離，導至失明。血管新生(angiogenesis)是正常生理變化中(如生長、傷口癒合)所必需有的過程，近年來科學家們也發現它和癌症的發展有密切的關係。當周圍的結締組織刺激後會分泌許多促使血管新生的物質(angiogenic substances)激活血管內皮細胞，而發生下列變化：1) 腫瘤周圍結締組織的分解破壞； 2) 內皮細胞增生； 3) 內皮細胞向分泌促使血管新生物質的地方移動； 4) 內皮細胞重新組合成新生血管。本實驗室另一方向在研究高濃度氧氣環境下，內皮細胞被誘導產生血管新生，用來做為病變致病機轉的研究。
最近發表之論文:
1. Liu SL, Li YH, Shi GY, Tang SH, Jiang SJ, Huang CW, Liu PY, Hong JS, Wu HL. 2009 Apr. Dextromethorphan reduces oxidative stress and inhibits atherosclerosis and neointima formation in mice. Cardiovasc Res. 82(1):161-169. (SCI)
2. Jiang SJ, Campbell LA, Berry MW, Rosenfeld ME, Kuo CC. 2008 Jul. Retinoic acid prevents Chlamydia pneumoniae induced foam cell development in a mouse model of atherosclerosis. Microbes Infect. 10(12-13):1393-1397. (SCI)
3. Jiang SJ, Kuo CC, Berry MW, Lee AW, Campbell LA. 2008 Apr. Identification and characterization of Chlamydia pneumoniae specific antigens which activateF production in RAW 264.7 murine macrophages. Infect. Immun. 76(4): 1558-1564. (SCI)
4. Kuo CC, Lee A, Jiang SJ, Yaraei K, Campbell LA. 2007 Jul. Inoculation of Chlamydia pneumoniae or Chlamydia trachomatis with ligand that inhibit attachment to host cells reduces infectivity in the mouse model of lung infection: implication for anti-adhesive therapy. Microbes Infect. 9(9):1139-1141. (SCI)
5. Jiang SJ, Lin TM, Shi GY, Eng HL, Chen HY, Wu HL. 2004 Sep. Inhibition of bovine herpesvirus-4 replication in endothelial cells by arsenite. Antiviral Res. 63(3):167-175. (SCI)
6. Jiang SJ, Lin TM, Shi GY, Eng HL, Chen HY, Wu HL. 2004 Jul-Aug. Inhibition of bovine herpesvirus-4 replication by arsenite through downregulation of the extracellular signal-regulated kinase signaling pathway. J Biomed Sci. 11(4):500-510. (SCI)
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