李展平老師實驗室介紹

專長：生物化學、分子生物學、微生物分子診斷學、奈米生物醫學

研究主題: 微生物分子診斷、奈米生物技術

說明:

1. Structure-Function of Nucleic Acids and Interactions between Nucleic Acid and Protein

One of our interests is to explore a very promising field of analyzing structural features of nucleic acids and nucleic acids-proteins interactions by molecular/biochemical analyses and direct image analyses using Scanning Probe Microscopy (SPM). We are in collaboration with Dr CC Wang’s group; they are specialized in the SPM technologies, at NCKU. Our molecular/ biochemical efforts are to clone the protein genes including RecA, SSB, T7 endonuclease I, pyrophosphatase, lambda integrase, P1 cre recombinase, Q Beta replicase, and many others, to express them in E. coli cells, to purify them with high purity and activity, and to analyze their functions in vitro and in vivo. DNA or RNA with specialized structural elements such as cruciform, H-DNA, attB/P site, pseudoknot will also be cloned or constructed. We expect these results (molecular/chemical and SPM) will provide us deeper insights into structural features of nucleic acids and even the dynamically structural changes under different conditions and also after interactions with proteins.

2. Molecular diagnosis of diseases caused by pathogens

For the past years, we have been focusing on the development of methods for the molecular diagnosis of diseases cause by pathogens, such as MTB, four blood-born viruses (HBV, HCV, HIV, and HTLV, assay performance see Figure 2) and many others. At the same period, we have also developed the method of using nano si-coated magnetic beads for the purification of viral DNA/RNA from clinical samples with low virus titer; cloned, expressed and purified PCR enzymes and related proteins to establish many PCR and RT-PCR assays. In addition, related technologies such as the use of nanogold for the detection of mutations and a new technology platform that would allow us to carry out high throughput screening of mutations and SNP, and also for genotyping analyses.