陳灝平老師實驗室介紹

專長：微生物基因體學、蛋白質化學、酵素與輔酶化學、化學生物學

研究方向:

　

1.基因體學與轉錄體學在臨床醫學的應用

2.B12代謝異常個案在分子醫學層次的探討

3.酵素活性分析

4.臨床檢驗方法的改進與開發

5.開發新型生物能源與光電材料

最近發表之論文:
1. HP Chen, M Chow, CC Liu, Al Lau, J Liu, and LD Eltis,* "Vanillin and vanillate catabolism in Rhodococcus jostil RHA1." (2011) (manuscript in preparation)

2. HP Chen, S Zhu, A Lau, AE Davis, SWM Eng, CG Howes, FH Crocker, KJ Indest, SJ Hallam, and LD Eltis,*"Complete genome sequence of Gordonia sp. KTR9." (2011) (manuscript in preparation)

3. KJ Indest,* CM Jung, HP Chen, D Hancock , C Florizone, LD Eltis,* FH Crocker “Functional Characterization of pGKT2, a 182-Kilobase Plasmid Containing the xplAB Genes, Which Are Involved in the Degradation of Hexahydro-1,3,5-Trinitro-1,3,5-Triazine by Gordonia sp. Strain KTR9.”(2010) Applied and Environmental Microbiology 76, 6329-6337.

4. CH Hsu,* and HP Chen* “Molecular modeling studies of the conserved B12-binding motif and its variants from Clostridium tetanomorphum glutamate mutase” (2010) Protein & Peptide Letters 17 759-764.

5. HP Chen,* HJ Hsu, FC Hsu, CC Lai, and CH Hsu, “The interactions between coenzyme B12 analogs and adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum.” (2008) FEBS Journal 275, 5960-5968.

6. CH Tseng, CH Yang, HJ Lin, C Wu, and HP Chen,* “The S subunit of D-ornithine aminomutase from Clostridium sticklandii is responsible for the allosteric regulation in D-alpha-lysine aminomutase.” (2007) FEMS Microbiology Letters 274,148-153.

7. YP Weng, WS Yang, FC Hsu, and HP Chen,* “Optimization of the overexpression of glutamate mutase S component under the control of T7 system by using lactose and IPTG as the inducers” (2006) Enzyme and Microbial Technology 38, 465-469.

8. FC Hsu, TJ Ho, CC Lai, CF Lin, and HP Chen,* “Cloning, sequencing, expression, and single-step purification of the adenosylcobinamide kinase/ adenosylcobinamide-phosphate guanylyltransferase (CobU) from Salmonella typhimurium ATCC 19585” (2005) Protein Expression and Purification, 42, 178-181.

9. HP Chen,* FC Hsui, LY Lin, CT Ren, and SH Wu, “Coexpression, purification, and characterization of the E and S subunits of coenzyme B12 and B6 dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli” (2004) European Journal of Biochemistry, 271, 4293-4297.

10. HP Chen,* FD Lung, CC Yeh, HL Chen, and SH Wu, “The role of the conserved histidine-aspartate pair in the ‘base-off’ binding of cobalamins”. (2004) Bioorganic and Medicinal Chemistry, 12, 577-582.

11. HP Chen,* SH Wu, YL Lin, CM Chen and SS Tsay, “Cloning, sequencing, heterologous expression, purification and characterization of adenosylcobalamin-dependent D-ornithine aminomutase from Clostridium sticklandii”. (2001) Journal of Biological Chemistry, 276, 44744-44750.

12. HP Chen,* CF Lin, YJ Lee, SS Tsay and SH Wu, “Purification and properties of ornithine racemase from Clostridium sticklandii” (2000) Journal of Bacteriology, 182, 2052-2054.